close
Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jan 30;110(2):49.
doi: 10.1007/s00128-023-03689-6.

Reproductive and Developmental Alterations on Zebrafish (Danio rerio) Upon Long-Term Exposure of Di-ethyl phthalate (DEP), Di-isononyl phthalate (DINP) and Di (2-ethylhexyl) phthalate (DEHP)

Shu Chuan Tsai  1 , Yu-Jen Tseng  2 , Su Mei Wu  3
Affiliations

Reproductive and Developmental Alterations on Zebrafish (Danio rerio) Upon Long-Term Exposure of Di-ethyl phthalate (DEP), Di-isononyl phthalate (DINP) and Di (2-ethylhexyl) phthalate (DEHP)

Shu Chuan Tsai et al. Bull Environ Contam Toxicol. .

Abstract

Di (2-ethylhexyl) phthalate (DEHP), di-ethyl phthalate (DEP) and di-isononyl phthalate (DINP) are all endocrine disrupting chemicals (EDCs) for organisms. However, little research has been done on the effects of long-term EDC exposure. The present study found that the zebrafish barely grew during the 7 months of DINP exposure. The fecundity rate (%) of female spawning was lower in the DEHP treatment by 4 months compared to other exposure groups. Zebrafish treated with 12.5-25.0 ppm of DEP for 4 months presented no spawning. Gonadal-somatic index (GSI) levels significantly decreased, and there were more oocytes in the atresia and peri-nucleus stage compared to the control group. In addition, the hatching rate of embryos were 71.02%, 56.92%, and 21.70% for females treated with DINP, DEHP and DEP, respectively. There were also abnormal craniofacial chondrogenesis development on 72 hpf embryos upon females treated with the three EDCs. In conclusion, long term exposure of DEHP, DINP, and DEP did not only affect the reproductive capacity of female zebrafish, but the 3 plasticizers also influence craniofacial cartilage development of its offspring.

Keywords: Cartilage; Ceratohyal; Phthalate; Plasticizers; Zebrafish.

PubMed Disclaimer

References

    1. Adeogun AO, Ibor OR, Imiuwa ME, Omogbemi ED, Chukwuka AV, Omiwole RA, Arukwe A (2018) Endocrine disruptor responses in African sharptooth catfish (Clarias gariepinus) exposed to di- (2-ethylhexyl)-phthalate. Comp Biochem Physiol 213C:7–18
    1. Barse AV, Chakrabarti T, Ghosh TK, Pal AK, Jadhao SB (2007) Endocrine disruption and metabolic changes following exposure of Cyprinus carpio to diethyl phthalate. Pesticide Biochem Physiol 88(36):42
    1. Boran H, Terzi S (2019) Bis (2-Ethylhexyl) phthalate induces DNA strand breaks and gene expression alterations in larval zebrafish Danio rerio. Toxicol Ind Health 35(520):529
    1. Bjȍrklund K, Strȍmvall AM (2009) Phthalates and nonylphenols in urban runoff:occurrence, distribution and area emission factors. Sci Total Environ 407:4665–4672 - DOI
    1. Chen JR, Wu SM, Tsai SC, Hsien FC, Huang CT (2020) Changes in vitellogenin and estrogen receptor expression and 17β-estradiol concentration in male juvenile tilapia can be used to evaluate endocrine disrupting chemicals. Comp Biochem Physiol 229C:108682

LinkOut - more resources