Bacterial growth at −15 °C; molecular insights from the permafrost bacterium <i>Planococcus halocryophilus</i> Or1

Mykytczuk, Nadia; Foote, Simon; Omelon, Christopher R.; Southam, Gordon; Greer, Charles W.; Whyte, Lyle G.

doi:10.1038/ismej.2013.8

The Isme Journal

2013

DOI: 10.1038/ismej.2013.8

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Bacterial growth at −15 °C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1

Nadia Mykytczuk

¹

,

Simon Foote

²

,

Christopher R. Omelon

³

et al.

Abstract: Planococcus halocryophilus strain Or1, isolated from high Arctic permafrost, grows and divides at À 15 1C, the lowest temperature demonstrated to date, and is metabolically active at À 25 1C in frozen permafrost microcosms. To understand how P. halocryophilus Or1 remains active under the subzero and osmotically dynamic conditions that characterize its native permafrost habitat, we investigated the genome, cell physiology and transcriptomes of growth at À 15 1C and 18% NaCl compared with optimal (25 1C) tempera… Show more

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Cited by 325 publications

(302 citation statements)

References 70 publications

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“…This is in agreement with previous reports of subzero growth and motility in marine broth suspensions (Huston et al, 2000;Junge et al, 2003;Marx et al, 2009). The decrease in Cp34H's metabolic rate with decreasing temperatures is consistent with general biological mechanisms and previous findings in psychrophilic bacteria (e.g., Junge et al, 2006;Piette et al, 2011;Mykytczuk et al, 2013). These results are also consistent with Junge and colleagues (2006), where active leucine incorporation was demonstrated down to −20°C in Cp34H.…”

Section: Discussion

supporting

confidence: 93%

“…Maximum [3H]-leu incorporation occurred at the 24 h time point for all temperatures and was greatest in the −1°C treatment and lowest at −10°C. The decrease in Cp34H's metabolic rate with decreasing temperatures is consistent with general biological mechanisms and previous findings in psychrophilic bacteria (e.g., Junge et al, 2006;Piette et al, 2011;Mykytczuk et al, 2013). At very low temperatures, low rates of metabolism may offset cellular and macromolecular damage in permafrost bacteria (Dieser et al, 2013), thereby allowing for survival over prolonged time frames .…”

Section: Discussion

supporting

confidence: 87%

See 1 more Smart Citation

Proteomics of C olwellia psychrerythraea at subzero temperatures – a life with limited movement, flexible membranes and vital DNA repair

Nunn

¹

,

Slattery

²

,

Cameron

³

et al. 2015

Environmental Microbiology

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…This is in agreement with previous reports of subzero growth and motility in marine broth suspensions (Huston et al, 2000;Junge et al, 2003;Marx et al, 2009). The decrease in Cp34H's metabolic rate with decreasing temperatures is consistent with general biological mechanisms and previous findings in psychrophilic bacteria (e.g., Junge et al, 2006;Piette et al, 2011;Mykytczuk et al, 2013). These results are also consistent with Junge and colleagues (2006), where active leucine incorporation was demonstrated down to −20°C in Cp34H.…”

Section: Discussion

supporting

confidence: 93%

“…Maximum [3H]-leu incorporation occurred at the 24 h time point for all temperatures and was greatest in the −1°C treatment and lowest at −10°C. The decrease in Cp34H's metabolic rate with decreasing temperatures is consistent with general biological mechanisms and previous findings in psychrophilic bacteria (e.g., Junge et al, 2006;Piette et al, 2011;Mykytczuk et al, 2013). At very low temperatures, low rates of metabolism may offset cellular and macromolecular damage in permafrost bacteria (Dieser et al, 2013), thereby allowing for survival over prolonged time frames .…”

Section: Discussion

supporting

confidence: 87%

Proteomics of C olwellia psychrerythraea at subzero temperatures – a life with limited movement, flexible membranes and vital DNA repair

Nunn

¹

,

Slattery

²

,

Cameron

³

et al. 2015

Environmental Microbiology

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…4 and 5 , and Table S4 ). The co-occurrence of these enzymes with their metabolic intermediates suggests maintenance of basal metabolic functions under subzero conditions, consistent with reports that cold-adapted microbes can sustain slow but measurable respiration and protein turnover even in frozen soils ( 73 , 74 ). This finding demonstrates that degradation processes begin before thaw-driven export, meaning that permafrost-derived carbon may already be partially processed before entering fjord systems.…”

Section: Discussion

supporting

confidence: 88%

Capacity of Arctic fjord sediments to degrade carbohydrates from permafrost active layer

Abuah

¹

,

Sipes

²

,

Buongiorno

³

et al. 2026

Microbiol Spectr

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…Similar observations have been reported in Arctic permafrost [21,26,27], where bacterial growth at sub-zero temperatures has been demonstrated for isolated strains [67][68][69]. As previously suggested [21,27,70], and despite the elevated energetic costs, permafrost microorganisms might retain activity because the risk of DNA degradation over time might select against spore formation as a long-term survival strategy [21,27,70].…”

Section: Discussion

supporting

confidence: 86%

Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps)

Perez-Mon

¹

,

Qi

²

,

Vikram

³

et al. 2021

Microbial Genomics

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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